vibrio
Banned
Registered: 28th Feb 01
Location: POAH
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Thinking about applying for this
Dear Ross
The post I have advertised will follow on from our recent publications
showing SNARE protein association with lipid raft domains. I still have to
finalise exactly what portion of the work will be taken on by the RA
employed, but it is likely to focus on SNARE proteins that function in
Glut4 vesicle exocytosis (in fat cells).
The direction we are taking is to generate mutant SNAREs which have either
an increased or decreased affinity for lipid rafts and to assess the
effects of these mutants on exocytosis. We are also using mutagenesis to
understand how SNARE proteins are targeted to rafts.
A major portion of the work taken on by the RA will investigate the
differential localisation of VAMP2 and cellubrevin in rafts. In fat cells
we find that >70% of VAMP2 is raft associated but only a small postion
(15%) of cellubrevin is in rafts. We would like to know whether this
difference is due to specific differences in the amino acid sequences of
these vSNAREs or whether it reflects the differential localisation of these
proteins in fat cells (VAMP2 is mainly found in GLUT4 vesicles, callubrevin
in the endosomal system). Other work will look at the effects of decreasing
the raft association of SNAP23 on GLUT4 vesicle exocytosis.
Techniques that would be useful to have experience in would come from the
following: gradient centrifugation, cell fractionation, SDS-PAGE,
immunoblotting, immunofluorescence, mammalian cell culture and transfection.
I think there should be no problems registering for a PhD (although the RA
may have to pay fees- although there may be ways around this).
Hope this helps- if you are interested you should note that the closing
date is tomorrow. Applications must be sent to Lesley Frew (details in
nature July 24th).
Luke
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Sooty
Banned
Registered: 9th Mar 03
Location: FLAP CENTRAL
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What is the pay?
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ajscorsa
Member
Registered: 30th Apr 02
Location: Perceton, North Ayrshire
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where is it. canny be assed reading
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Scott
Member
Registered: 11th Mar 01
Location: Kilmarnock,Scotland
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u need to get a new job rossco
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ajscorsa
Member
Registered: 30th Apr 02
Location: Perceton, North Ayrshire
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scott you need to get a job period. 
you sent them cv's yet
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Scott
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Registered: 11th Mar 01
Location: Kilmarnock,Scotland
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quote:
Originally posted by ajscorsa
scott you need to get a job period.
you sent them cv's yet
no i got few ideas atm which im working on , business wise
plus can live on wot ive got for a good few yrs  
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ajscorsa
Member
Registered: 30th Apr 02
Location: Perceton, North Ayrshire
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 ya cunt wae all your money gimmie some
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Ian
Site Administrator
Registered: 28th Aug 99
Location: Liverpool
User status: Online
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Mad head 
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Fraser Young
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Registered: 26th Dec 02
Location: Dundee City Drives: 58 Impreza WRX
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I think there should be no problems registering for a PhD (although the RA
may have to pay fees- although there may be ways around this).
What an inspecific piece of information!
Sounds interesting tho, where (geographiacally) is it?
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vibrio
Banned
Registered: 28th Feb 01
Location: POAH
User status: Offline
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Glasgow uni. pay 18-22K
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Tom
Member
Registered: 3rd Apr 02
Location: Wirral, Merseyside
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Just read that ...I read it 'blah blah blah bladdy bladdy blah'
I get paid nearly as much as your lower end there...n i aint got no quals
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vibrio
Banned
Registered: 28th Feb 01
Location: POAH
User status: Offline
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the pay sucks but then that is the bottom rung of the ladder
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