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Author just incase you wanted to do your own SARS test
vibrio
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Registered: 28th Feb 01
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15th Apr 03 at 14:23   View User's Profile U2U Member Reply With Quote
here are the PCR primers you require to perform your PCR. using a Lightcycler will give you results in 2hours.

BNIoutS/BNoutAs: sense ATGAAT TAC CAA GTC AAT GGT TAC
antisense CAT AAC CAG TCG GTA CAG CTA C
FraGment lenGth 195 bp

BNIinS/BNIAs: sense GAA GCT ATT CGT CAC GTT CG
antisense CTGTAGAAA ATC CTA GCT GGA G
Fragment length 110 bp

SAR1s/SAR1as: sense CCT CTC TTG TTC TTG CTC GCA
antisense TAT AGT GAG CCG CCA CAC ATG
Fragment length 150 bp

Amplicon sequence (BNI-1, Bernhard-Nocht Institute, Hamburg, Germany)
TACCGTAGACTCATCTCTATGATGGGTTTCAAAATGAATTACCA
AGTCAATGGTTACCCTAATATGTTTATCACCCGCGAAGAAGCTAT
TCGTCACGTTCGTGCGTGGATTGGCTTTGATGTAGAGGGCTGTCAT
GCAACTAGAGATGCTGTGGGTACTAACCTACCTCTCCAGCTAGGA
TTTTCTACAGGTGTTAACTTAGTAGCTGTACCGACTGGTTATGTTGA
CACTGAAAATAACACAGAATTCACCAGAGTTAATGCAAAACCTCC
ACCAGGTGACCAGTTTAAACATCTTATACC

[Edited on 15-04-2003 by vibrio]
Tommy
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Location: Essex, Colchester
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15th Apr 03 at 14:25   View User's Profile U2U Member Reply With Quote
Right
someone
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Location: Surrey
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15th Apr 03 at 14:27   View User's Profile U2U Member Reply With Quote
ill try that straight away
vibrio
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15th Apr 03 at 14:30   View User's Profile U2U Member Reply With Quote
oh a light cycler will set you back the best part of 50K
J1
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Registered: 9th May 02
Location: Bedfordshire
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15th Apr 03 at 14:32   View User's Profile U2U Member Reply With Quote
A light tester, i think someone was selling one of those in the classified and wanted section LOL
corb
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Registered: 24th Apr 02
Location: Calgary, Alberta, Canada
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15th Apr 03 at 23:03   View User's Profile U2U Member Reply With Quote
i did, not looking good, someone call me an ambula
Tommy
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Location: Essex, Colchester
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15th Apr 03 at 23:10   View User's Profile U2U Member Reply With Quote
nce
LoudandProud
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Location: Stanway, Essex
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15th Apr 03 at 23:13   View User's Profile U2U Member Reply With Quote
did i miss the whole point of this post
CorsaLad
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Registered: 25th Sep 01
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15th Apr 03 at 23:27   View User's Profile U2U Member Reply With Quote
something to do with genetics?
Lar 23
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Location: Bedfordshire
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16th Apr 03 at 00:00   View User's Profile U2U Member Reply With Quote
Looks like DNA coding fuck all use to me though
Joff
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Registered: 17th Oct 00
Location: Cambridgeshire
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16th Apr 03 at 07:07   View User's Profile U2U Member Reply With Quote
Just got to wait 2 hours now...
Demo
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Registered: 27th Sep 01
Location: south wales Drives: astra sri ecoflex
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16th Apr 03 at 08:39   View User's Profile U2U Member Reply With Quote
im gonna have sars on the weekend if the weathers nice
Steve
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Registered: 30th Mar 02
Location: Worcestershire Drives: Defender
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16th Apr 03 at 08:41   View Garage View User's Profile U2U Member Reply With Quote
quote:
Originally posted by Demo
im gonna have a wank on the weekend if the weathers nice


thats nice but really u shuld come to my bbq instead

[Edited on 16-04-2003 by Steve106GTi]
jay@ffwd
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Registered: 14th Jan 03
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16th Apr 03 at 08:41   View User's Profile U2U Member Reply With Quote
quote:
Originally posted by vibrio
oh a light cycler will set you back the best part of 50K


I know a man...
Back of a lorry
Demo
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16th Apr 03 at 08:43   View User's Profile U2U Member Reply With Quote
quote:
Originally posted by Steve106GTi
quote:
Originally posted by Demo
im gonna have a wank on the weekend if the weathers nice


thats nice but really u shuld come to my bbq instead

[Edited on 16-04-2003 by Steve106GTi]


maybe i will and then i can blow in your face
vibrio
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16th Apr 03 at 08:46   View User's Profile U2U Member Reply With Quote
this is a thread hijack.. please piss off

 
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